https://file.aiquickdraw.com/mathbot/file/17272315518637cy91cnv.jpg

It looks like you’ve uploaded an image from a Colony Forming Unit (CFU) calculation simulation. The image shows the following data for the calculation of CFU/mL:

- **Number of Colonies**: 2
- **Volume used for Spreading**: 0.1 mL
- **Dilution Factor**: 10³

The result displayed is:
\[ 2.0 \times 10^3 \, \text{cfu/mL} \]

This result is calculated using the formula:
\[
\text{CFU/mL} = \frac{\text{Number of Colonies}}{\text{Volume used for Spreading} \times \text{Dilution Factor}}
\]

Given:
\[
\text{Number of Colonies} = 2, \quad \text{Volume} = 0.1 \, \text{mL}, \quad \text{Dilution Factor} = 10^3
\]
\[
\text{CFU/mL} = \frac{2}{0.1 \times 10^3} = \frac{2}{100} = 0.02 \times 10^3 = 2.0 \times 10^3 \, \text{cfu/mL}
\]

Would you like further details on this calculation or have any questions? 

Here are 5 related questions:
1. What does CFU represent in microbiology?
2. How does dilution factor affect the CFU calculation?
3. What would happen if you used a different volume for spreading?
4. How can you improve accuracy when counting colonies in a Petri dish?
5. What are the limitations of the CFU method in microbial counting?

**Tip**: Always ensure proper dilution and spreading technique to get accurate colony counts in microbiology experiments.

How to calculate CFU/mL given 2 colonies, a spreading volume of 0.1 mL, and a dilution factor of 10³?

Learn how to calculate CFU/mL using the number of colonies (2), volume used for spreading (0.1 mL), and dilution factor (10³). This detailed guide explains the step-by-step calculation for a result of 2.0 x 10³ CFU/mL, suitable for microbiology students.

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